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qARTA Bio Products

q•Hot Taq Master Mixes

Catalog No. Description Volume Size
QHMM15-01 q•Taq Hot Master Mix RTL, 1.5mM MgCl2 1 mL 250 reactions
QHMM20-01 q•Taq Hot Master Mix RTL, 2.0mM MgCl2 1 mL 250 reactions
QHMM25-01 q•Taq Hot Master Mix RTL with Proofreading, 2.5mM 1 mL 250 reactions
QHMM30-01 q•Taq Hot Master Mix RTL with Proofreading, 3.0mM 1 mL 250 reactions
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Product Detail
Reaction Mix
Protocol
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q•Hot Taq Master Mixes are each optimized ready-to-use solutions for Hot Start PCR. They contains q•Hot Taq DNA polymerase, dNTPs, and MgCl2. Only template, primers, and water need to be added.

Recommended Reaction Mix

Recommended PCR Mix:

Component Concentration Volume Final Concentration
q•Taq Master Mix 5X 4 µL 1 X
Primer, forward 10 pmol/µl 0.2 - 0.6 µL 100 - 300 nM
Primer, reverse 10 pmol/µl 0.2 - 0.6 µL 100 - 300 nM
Template DNA variable variable 5 - 50 ng/µL
Nuclease-free water variable variable
Total 20 µL

For complete usage instructions download full User Protocol.

Recommended Instrument Protocol

Recommended Instrument Protocol:

Cycle Step Temperature Time Cycles
Initial denaturation 95°C 12 - 15 min 1
Denaturation 95°C 10 - 20 s 25 - 30
Annealing 54 - 66°C 30 - 60 s
Extension 72°C 20 s - 4 min
Final Extension 72°C 5 - 10 min 1

For complete usage instructions download full User Protocol.

  • Environmentally green: No shipping on ice required. Reduces packaging waste by 75%!

  • Nuclease-free Water Included: All mixes include sufficient RNase and DNase Free water for recommended reaction volume.