q•Blue is an inert colorant for coloring one or more components of a PCR or qPCR reaction, such as the DNA template or master mix, for visible detection. It does NOT interfere with PCR amplification or the detection of PCR product using fluorescent dyes.
Recommended Protocol
Recommended Usage Protocol:
Mix Concentration
Protocol
2X
Add 18 μL of q•Blue with 1 mL of 2X PCR or qPCR master mix
5X
Add 45 μL of q•Blue with 1 mL of 5X PCR or qPCR master mix
For use with ABI instruments, additional instrument settings may be required. Please contact technical.support@qartabio.com or your local sales representative for more information.