FAQ |
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PCR Technical Support |
Q: | How much qARTA master mix do I need in my reaction? |
Details of the recommended master mix can be found in each of our User Protocols.
For a 5X mix and a 20uL reaction volume use 20 µL / 5 = 4 µL. |
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Q: | Why don't qARTA qPCR mixes come in 2X? |
The 5X concentration is an integral part of being able to ship our products without ice to save transportation time and costs. |
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Q: | My Cq value when using qARTA's EvaGreen mix is higher than when using my standard SYBRGreen mix? |
In melt curve analysis, qARTA EvaGreen mixes usually show a higher melting temperature because EvaGreen has a different chemistry than SYBRGreen. The Tm is usually 2°C higher. |
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Q: | The qARTA PCR master mix had a low yield or no PCR product at all. Why? |
This may be due to a variety of reasons including:
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